Molecular Biologist

Germany

4 years experience

Birthday:

Nationality:
Italian

46919196

University education

Timeframe
Degree / faculty / university
Study areas

2008-01 – 2012-03

completed

Ph.D.
Biology
, Italy

Biology and cellular biotechnologies

2005-01 – 2007-07

completed

Master's Degree
Marine Biology
, Italy

Marine Biological Resources

2001-09 – 2005-01

completed

Bachelor of Science
Biology
, Italy

Biology and marine Ecology

Professional experience

Timeframe
Occupation / company
Tasks

2013-01 – 2017-01

PostDoc
, United Kingdom

- Optimized RNA isolation and library preparation protocols from single blastomeres in X. tropicalis for single cell RNA sequencing
- Mastering NGS technology such as RNA-seq, DNA-Seq, ATAC, HI-C
- Trained and supervised PhD and Master students on basic molecular techniques, NGS methods and experimental design
- Published peer-reviewed papers, presented data at international conferences and internal seminars, and currently writing book chapter about RNA-Seq in Xenopus
- Ordered supplies and managed day to day running of lab
- Responsible for the organisation of the new laboratory

Training and development

Timeframe
Degree / institute
Content

Further training

2016-12 – 2016-12

completed

Advanced Management Skills

, United Kingdom

Further training

2016-06 – 2016-07

Next-Generation Sequencing Data Analysis

, Germany

Further training

2013-04 – 2013-04

Cell & Developmental Biology of Xenopus

, United States

Further training

2010-05 – 2010-05

Experimental Developmental Biology of Marine Invertebrates

, France

Publications

Date
Type of publication / publisher
Title / content

Author / creator

2015

Article in a journal

Co-author / co-creator

2014

Article in a journal


Co-author / co-creator

2014

Article in a journal

Author / creator

2013

Article in a journal

Co-author / co-creator

2012

Article in a journal

Author / creator

2011

Article in a journal

Co-author / co-creator

2011

Article in a journal

Co-author / co-creator

2011

Article in a journal

Co-author / co-creator

2010

Article in a journal

Language ability

Language
Level
Exam / certificate
Italian
Mother tongue

–

English
Fluent (C1)

–

German
Advanced (B1)

–

Other skills

Key professional skills
Driver licenses
Interests, hobbies, personal

TECHNICAL
- DNA and RNA isolation from water, soil and animal tissues
- Libraries preparation for Illumina platform (Single Cell, RNA, DNA, ATAC, HI-C)
- Quality assessment of purified nucleic acids (Bioanalyzer‐ nanodrop)
- Sanger sequencing
- Gene expression analyses (RT-PCR, qPCR)
- Gene expression modulation (Morpholino - CRISPR/Cas9 – mRNA)
- Gene cloning (Standard, gateway)
- Histology tissue processing
- Histochemical assays (IHC-ISH-FISH)
- Protein isolation, Western Blotting and Immunoprecipitation
- Genotoxicity and enzymatic assays

COMPUTATIONAL
- Windows and MacOSX operative system
- Basic knowledge of Linux shell scripting
- MS Office package, Adobe Acrobat, Adobe Photoshop
- Knowledge of NGS data analysis tools (FastQC –cutadapt- Bowtie2-STAR-IGV)

  • Automobile

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