TECHNICAL
- DNA and RNA isolation from water, soil and animal tissues
- Libraries preparation for Illumina platform (Single Cell, RNA, DNA, ATAC, HI-C)
- Quality assessment of purified nucleic acids (Bioanalyzer‐ nanodrop)
- Sanger sequencing
- Gene expression analyses (RT-PCR, qPCR)
- Gene expression modulation (Morpholino - CRISPR/Cas9 – mRNA)
- Gene cloning (Standard, gateway)
- Histology tissue processing
- Histochemical assays (IHC-ISH-FISH)
- Protein isolation, Western Blotting and Immunoprecipitation
- Genotoxicity and enzymatic assays
COMPUTATIONAL
- Windows and MacOSX operative system
- Basic knowledge of Linux shell scripting
- MS Office package, Adobe Acrobat, Adobe Photoshop
- Knowledge of NGS data analysis tools (FastQC –cutadapt- Bowtie2-STAR-IGV)