Technical
1. Molecular Techniques: Isolation of DNA and RNA from plant, bacteria and fungus, isolation of plasmid DNA, PCR (Rep-PCR, Multiplex PCR, Inverse PCR, Touch down PCR), targeted genome editing – CRISPR/Cas9, RT – PCR, gene isolation and promoter isolation techniques (RAGE & RACE), cloning, vector construction, genome walking PCR, real time PCR (qPCR), SSH, cDNA library construction, protein isolation and purification, SDS PAGE, southern and western blotting, sanger and next generation sequencing.
2. Tissue Culture and Transgenic techniques: Callus culture (Sugarcane, Wheat, Rice and Tobacco), particle bombardment and Agrobacterium mediated transformation (Sugarcane, Rice, Tobacco, Petunia, Tomato, N.benthamiana and BY2 cells), celite-503 abrasion mediated transformation (Tobacco, Petunia, Tomato), protoplast isolation.
3. Bioinformatics skills: Data mining (NCBI, E M B L, SW ISSPROT), multiple sequence alignment (ClustalW, ClustalX), primer analysis (Fast PCR, Net Primer), primer synthesis (degenerate & gene specific), Nex t generation sequence analysis, proficient in statistical analysis and software (STATISTICA & Open stat).
4. Microbiological Techniques: Isolation and identification of bacteria and fungus, culturing of microorganisms, staining techniques, conventional fluorescent/confocal microscopy, water and milk quality test, antibiotic sensitivity test, ELISA, immunological techniques.
Managerial
1. Project: Design and management of research projects, strong organizational abilities and time management skills, excellent written and verbal communication, grant and manuscript writing, proficiency working as individual and in a team, attention to detail.
2. Personnel Training: Supervising and teaching masters and bachelors students in their research projects, scientific data presentation to lay personnel.
